PCR buffers공급업체: VWR Chemicals
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Buffers PCR Buffers
An optimal buffer system is critical for the performance of successful PCR. VWR® Taq DNA Polymerase kits generally include two different buffers, Key Buffer and Extra Buffer, which are suited for different PCR needs. All buffers contain Tris and 15 mM MgCl2 (1,5 mM MgCl2 final concentration). Additional MgCl2 for easy optimisation is included in a separate vial.
Key Buffer: Key Buffer (NH4+) gives a superior amplification signal (high yield) and minimises the need for optimisation of the Mg2+ concentration or the annealing temperature in most primer-template systems.
Extra Buffer: Extra Buffer is the traditional potassium (K+) buffer. Extra Buffer promotes high specificity. Careful optimisation of primer annealing temperatures and Mg2+ concentrations may be required.
Combination Buffer: Combination Buffer is a proprietary mixture of K+ and NH4+. It combines high specificity with good product yield and high tolerance to optimisation of primer annealing temperatures and Mg2+ concentrations due to its balanced ammonium-potassium formulation.
Buffers for GC-rich templates: Combined with VWR® TEMPase the GC buffers promote excellent amplification results with targets of varying degrees of GC content. This confirms that the choice of buffer is crucial for any successful amplification.