VisiGlo™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.
- Highly sensitive substrates save precious protein samples and antibodies
- Low background without compromising signal intensity
- Quantitative with broad dynamic range
- Long lasting signal
- Compatible with CCD and X-ray film imaging systems
VisiGlo™ Select HRP chemiluminescent substrate kit combines a broad linear dynamic range and attomolar sensitivity to enable quantitation of low abundance targets labelled with horseradish peroxidase (HRP). Low intensity signals undetectable with competing chemiluminescent substrates may be revealed as well, because background levels remain low even for lengthy exposure times. The signal generated by substrate incubation is stable for hours and may be captured by conventional X-ray film, CCD and fluorescence-based imaging systems.
VisiGlo™ Prime HRP chemiluminescent substrate kit delivers a wide dynamic range of HRP detection that is linear over three orders of magnitude, enabling accurate and quantitative comparison of proteins, especially when combined with CCD imaging. Highly sensitive VisiGlo Prime is ideal for detection of low abundance proteins, with sensitivity down to attomolar levels. High abundance proteins are detected without exhibiting substrate depletion, an important feature for simultaneous detection of low and high abundance proteins in a single exposure. The chemiluminescent emission from VisiGlo™ Prime is highly stable, lasting hours after substrate incubation to allow time for multiple exposures to obtain optimal blotting images.
High sensitivity and signal-to-noise ratio allow for target detection with minimal consumption of valuable protein and antibody reagents, thereby lowering experimental costs.